Eppendorf 5402 Manual Jun 2026

Over time, ice buildup occurs along the chamber walls due to the refrigeration system. Leave the centrifuge lid open overnight with the power off to let the ice melt. Wipe up the pool of water completely before restarting.

The Eppendorf 5402 Refrigerated Microcentrifuge is a legendary piece of laboratory equipment, known for its robust construction, precise cooling capabilities, and reliability in molecular biology and biochemistry labs. Although it has been superseded by newer models, the 5402 remains a workhorse in many facilities.

I need to verify the exact Fast Cool function steps and maintenance schedule for the F-45-18-11 rotor . eppendorf 5402 manual

Ensure the device is placed on a stable, level surface. The manual provides detailed instructions on assembling and installing the centrifuge.

Before operating the Eppendorf 5402, ensure the instrument is placed in an environment that allows it to function safely and efficiently. Laboratory Placement Over time, ice buildup occurs along the chamber

The Eppendorf Centrifuge 5402 is a classic, high-performance refrigerated microcentrifuge designed for quick-spin applications, pelleting of macromolecules, and temperature-sensitive biological separations. Known for its robust build quality and exceptional reliability, this legacy instrument remains a staple in molecular biology, biochemistry, and clinical laboratories worldwide.

If samples are spilled, clean the rotor immediately. The F-45-18-11 rotor is susceptible to corrosion from aggressive chemicals. Ensure the device is placed on a stable, level surface

A rotary dial to set run duration. Turning it counterclockwise past 1 minute activates the continuous "Hold" mode.

| Symptom / Error Code | Possible Cause(s) | Recommended Solution(s) | | :--- | :--- | :--- | | | Power outage, device not supplied with voltage | Check mains/power fuse of the lab and the mains/power cord. | | Cannot Open Lid | Rotor still running, mains/power outage | Wait for rotor to stop, then open. If power outage, use emergency release. | | Device Does Not Start | Centrifuge lid is open | Close centrifuge lid. | | Device Shakes on Start | Rotor is asymmetrically loaded / imbalanced | Stop centrifugation. Load rotor symmetrically, then restart. | | Temperature Display Flashes | Device hasn't reached set temperature (>3°C difference) | Wait for device to reach set point, or pre-temper with FastTemp function. | | Error 02 (Rotor Shuts Down) | Rotor imbalance, over-speed detection, or sensor issue | Check loading balance; contact technical support if issue persists. | | "balan." Error | Rotor imbalance or missing leveling foot | Check sample balance and inspect centrifuge feet for stability. | | Error Code A | Temperature sensor failure | Replace temperature sensor; contact Eppendorf for parts. |

If the unit loses power, the electronic lock will not disengage. Locate the emergency lid release hole on the side or bottom of the centrifuge, insert a thin tool (like an Allen wrench), and pull to manually open the latch.